Dilute ammonia solution preparation
H2S generated from a Kipps apparatus ( ~42g/l)ĭissolve 12.7 g of iodine in a solution of 20g of pure KI in 30 ml of water, and dilute to 1 liter with water.(0.05m)ĭissolve 95 g of the salt in 1 liter of water (0.25M)ĭissolve 62 g of the salt in 1 liter of water (0.25M) HCl (0.5M)ĭissolve 140 g of the salt in 1 liter of water containing 7 ml of conc. Sulphuric acid.(0.5M)ĭissolve 135 g of the hydrated salt in 1 liter of water containing 20ml of conc.
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(0.15M)ĭissolve 125 g of the salt in 1 liter of water containing 3ml of conc. Preserve in a dark coloured bottle.(6.8g/l)ĭissolve 44 g of the salt in 1 liter of water. Saturate 250ml of water with chlorine.the chlorine may be prepared by dropping conc.
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Shake 3 g of the salt with 1 liter of water, filter and decant the saturatrd solution after several hours (0.015M) Add more bromine if necessary to a slight excess.ĭissolve 55 g of the hydrated salt in 1 liter of water (0.25M) (1M)ĭissolve 71 g of the crystaline salt in 1 liter of water.(0.5M)ĭissolve 132 g of the salt in 1 liter of water.(1M)ĭissolve38 g of the salt in 1 liter of water (0.5M)ĭissolve 61 g of the salt in 1liter of water.(0.25M)Ī saturated aqueous solution is prepared by shaking 35g or 11ml liquid bromine with water. (2M)ĭissolve 169 g of the salt in 1 liter of solution (3M).ĭissolve 80 g of the salt in 1 liter of water. Dissolve 192 g of the salt in a mixture of 140ml conc.Īmmonia solution and water to make up 1 liter of solution. The commercial salt is a mixture of bicarbonateĪnd carbamate. One enzyme activity unit is defined as the amount of enzyme that oxidizes 1 µmol of acetaldehyde per minute when the test is conducted under the conditions described herein.If reagent not found in this list check the alphabetical listing below.ĭissolve 231 g of the salt in 1 liter of water. Calculate the change, D A, in absorbance per minute for the solution obtained from the Assay preparation, starting at the point when the absorbance and time relationship becomes linear. Stopper the cells, and determine the absorbance of the solution obtained from the Assay preparation at a wavelength of 340 nm, using the solution obtained from the reagent blank as the reference. Add 0.01 mL of acetaldehyde solution (0.3 in 100) to each cell, and mix. Stopper the cells, and allow to stand for 2 minutes at 25 ± 1. Add 2.5 mL of pH 9.0 buffer, 0.2 mL of -NAD solution, and 0.1 mL of pyrazole solution (0.68 in 100) to each cell, and mix. Pipet 0.1 mL of water into a second 1-cm spectrophotometric cell to provide the reagent blank. Pipet 0.1 mL of the Assay preparation into a 1-cm spectrophotometric cell. Dissolve an accurately weighed quantity of -nicotinamide adenine dinucleotide ( -NAD) in water to obtain a -NAD solution having a known concentration of about 20 mg per mL. Dissolve 3.3 g of potassium pyrophosphate, 15 mg of dithiothreitol, and 40 mg of edetate disodium in 70 mL of water, adjust with citric acid monohydrate solution (2.1 in 10) to a pH of 9.0 ± 0.1, dilute with water to 100 mL, and mix to obtain a pH 9.0 buffer. Use this solution as the Assay preparation. Transfer about 20 mg, accurately weighed, to a 200-mL volumetric flask, dissolve in 1 mL of water, dilute with an ice-cold solution of bovine serum albumin (1 in 100) to volume, and mix.